| Eppendorf Perfectpure C-18 Pipet Tips - Model 957010008 - Pack Of 96 |
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- volume: 10 -|- , ul. eppendorf no.: 957010008.
- Manufacturer: Eppendorf - Model 957010008 - Unit: Pack of 96
- Eppendorf PerfectPure C-18 Pipet Tips
Eppendorf PerfectPure C-18 Pipet Tips - Model 957010008 - Pack of 96 : These tips have a flow-through C-18 chromatographic material located inside. Femtomole and picomole quantities of peptides from proteolytic digests can be purified, desalted, and concentrated. An easy purification procedure, following mass spectrometry analysis and other downstream applications, results in no unspecific generation of mass peak adducts by salts and buffer contaminants, higher signal intensities, improved sequence coverage, unbiased identification of peptide fragments, and more reproducible spectra. The protocol is straightforward and simple: the aqueous sample mixture is aspirated once or several times through the binding matrix; peptides are bound to the C-18 particles by hydrophobic interaction. Multiple washings of tip with water will remove salts and other contaminants, while the peptides remain bound to the reversed-phase matrix. The purified peptides are eluted by one or more pipetting steps using minimum amounts of organic solvents, such as acetonitrile and/or n-propanol. Applications include sample preparation for MALDI and ESI mass spectrometry (desalting, purification, and concentration of peptide mixtures after protein digests), spotting sample directly onto a sample plate together with the MALDI matrix solution, elution for ESI MS analysis, or other downstream applications. Product features 10uL pipet tip with a flow-through matrix at its outlet, innovative manufacturing technology resulting in very good reproducibility, compatibility with step-gradient elution for peak suppression of smaller fragments, optimum interaction between peptide and matrix by ideal flow behavior, and protocols for electronic single-channel and multichannel pipettors. Tips fit Eppendorf pipettors and those from most other suppliers.
Shipping Weight: 4lb
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